Description
Biosafety Level 4 is required for work with dangerous and exotic agents that pose a high individual risk of aerosol-transmitted laboratory infections and life-threatening disease that is frequently fatal, for which there are no vaccines or treatments, or a related agent with unknown risk of transmission. Agents with a close or identical antigenic relationship to agents requiring BSL4 containment must be handled at this level until sufficient data are obtained either to conform continued work at this level, or re-designate the level.
Practices
Laboratory staff must have specific and thorough training in handling extremely hazardous infectious agents. Laboratory staff must understand the primary and secondary containment practices, containment equipment, and laboratory design characteristics. All laboratory staff and supervisors must be competent in handling agents and procedures requiring BSL4 containment. The laboratory supervisor in accordance with institutional policies controls access to the laboratory.
Primary Barriers & Safety Equipment
All procedures within the facility with agents assigned BSL4 are conducted in the Class III biological safety cabinet or in Class I or II biological safety cabinets used in conjunction with one-piece positive pressure personnel suits ventilated by a life-support system.
Facilities - Second Barriers
The maximum containment facility consists of either a separate building or a clearly demarcated and isolated zone within a building.
For additional detailed standards refer to the NIH Guidelines and the BMBL.